Application

DNA Molecular Weight Marker III provides accurate sizing of fragments over a broad range of sizes. The fragments have 5′-protruding ends and can be labeled with radioactive nucleotides (e.g., [³²P]-dTTP or [³²P]-dGTP) by standard filling-in reactions. End-labeling reactions can be performed with a radioactive or nonradioactive dideoxynucleotide (e.g., DIG-11-ddUTP) and Terminal Transferase.

DNA Molecular Weight Marker for agarose gels; simplifies accurate molecular weight determination of double-stranded DNA fragments generated by: Restriction digests PCR RT-PCR

General description

The bacteriophage λDNA is fragmented in a restriction digestion with EcoR I and Hind III endonuclease.The product is also available as DNA Molecular Weight Marker III, Digoxigenin-labeled.Size Range: 0.12 to 21.2 kbp

ContentsReady-to-use solution, 250 µg/ml, in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working concentration: 1 to 3 mg/ml bufferWorking solution: Recommended solvent is 25 mM Tris/HCl, 300 mM NaCl, 1mM EDTA pH 8.0.Product is stable in 5 mM urea and 0.1% SDS (w/v).Storage conditions (working solution): 2 to 8 °COnce thawed, store at 2 to 8 °C.

Sequence

The digestion reaction results in thirteen double-stranded DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 125, 564, 831, 947, 1375, 1584, 1904, 2027, 3530, 4268, 4973, 5148, and 21226 as determined by computer analysis of the λDNA sequence.Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.

Storage and Stability

Store at -15–-25 °C. (until use)

Unit Definition

50 µg = 1A₂₆₀ unit